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DyeTox13 Green C-2 Azide for Differentiation of Viable and Nonviable Bacteria Using PCR

Technology #2018-040

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PCR detection of viable bacteria that exhibit metabolic activity
Categories
Researchers
Bae Sung Woo
Managed By
Dr Ler Ser Yeng
Manager (65)66016248
Files and Attachments
Tech Offer [PDF]

MARKET OPPORTUNITY 

The bacteriological testing market was USD 9.58 billion in 2017, and is projected to reach USD 13.98 billion by 2022, at a compound annual growth rate of 7.8%. Culture methods require 4-5 days to obtain presumptive positive or negative results and can take up to 7 days, depending on the biochemical and serological confirmations. The current dyes used for viability PCR target cells with compromised membranes. Not all inactivation methods disrupt the cell membrane so not all nonviable cells have compromised membranes. As a result, the estimation of the viable bacteria is inaccurate and bacterial cells can remain visibly intact and impermeable to the dyes for hours or days after the viability is lost. 



TECHNOLOGY 
This is a dye that can selectively detect the cells that exhibit metabolic activity. Hence, it has the innate ability to detect inactivation under conditions that do not impact membrane permeability. 

CATEGORY 
Reagents 

STAGE OF DEVELOPMENT 
TRL2 

APPLICATIONS 
• Probiotic detection 
• Microbiological Testing of Water 
• Clinical diagnosis 
• Food Safety Testing 

ADVANTAGES 
• Accurate estimation of viable bacteria 
• Cheap 
• Easy to use 

STATUS 
Patent pending