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Mouse anti HLA-B5801 (clone 1F8)

Technology #2017-298-170720

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PRODUCT NAME Anti – HLA-B*58:01 Antibody (CLONE 1F8)

BACKGROUND INFORMATION An antibody that reacts with an antigen from a genetically different individual of the same species.

PRODUCT DESCRIPTION This antibody is suitable for allergy studies in transfusion or transplant. It is highly specific for HLA-B* 58:01 protein.

FORMAT 100µg of lyophilized, purified antibody. Reconstitute to 100µl of distilled H2O for 1µg/µl. It contains no additives

HOST Mouse

CLONALITY Monoclonal

ISOTYPE IgG1, mouse chimeric IgG1

REACTIVITY/SPECIFICITY This antibody is highly specific for HLA-B* 58:01 protein.

APPLICATIONS ELISA, FACs.

RECONNENDED STARTING DILUTIONS ELISA: 1:1000. FACs: 1:500. Optimal dilution has to be determined by the user.

STORAGE Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°C  for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

STABILITY Minimum 1 year from reception date.

REFERENCES 1. Chan JHS, Chua YL, Peh HY, Jovanovic V, Gascoigne NRJ, Wong WSF, Chew FT, Hanson BJ, Kemeny DM, MacAry PA. Molecular engineering of a therapeutic antibody for Blo t 5-induced allergic asthma. J Allergy Clin Immunol. 2017 May;139(5):1705-1708

LIMITATIONS This product has to be used for research purposes only.

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