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Human Anti-15 Keto Cholestane (Oxidized Cholesterol)

Technology #2017-204-170507

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Tech Offer 2017-204 Antibodies [PDF]

Anti-15 Keto Cholestane HU #170507

PRODUCT NAME Anti – 15 Keto Cholestane Antibody 

BACKGROUND INFOMATION Oxysterols represent a family of lipids implicated in diverse human diseases where Mab-based detection assays could have a profound effect on their utility as clinical biomarkers. The antibody is targeting a lipid implicated in multiple sclerosis and Autoimmune Encephalomyelitis (EAE).. This antibody may be used for utilizing phage display to isolate highly specific antibodies against poorly immunogenic, small molecule lipids. 

PRODUCT DESCRIPTION This antibody is suitable for lipids and glycolipids study. It is highly specific for 15- Keto Cholestane and shows little or no binding activity for other closely related oxystrols. 

FORMAT 100µg of lyophilized, purified antibody. Reconstitute to 100µl of distilled H2O for 1µg/µl. It contains no additives. 

HOST Human 

CLONALITY Monoclonal 

ISOTYPE IgG1 

REACTIVITY/ SPECIFICITY This antibody is highly specific for 15- Keto Cholestane and shows little or no binding activity for other closely related oxystrols. 

APPLICATIONS ELISA 

RECOMMENDED STARTING DILUTIONS ELISA: 1:100. Optimal dilution has to be determined by the user. 

STORAGE Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. 

STABILITY Minimum 1 year from reception date. 

REFERENCES 1. Islam MO1, Lim YT, Chan CE, Cazenave-Gassiot A, Croxford JL, Wenk MR, Macary PA, Hanson BJ.Generation and characterization of a novel recombinant antibody against 15-ketocholestane isolated by phage-display. Int J Mol Sci. 2012;13(4):4937-48. 
LIMITATIONS This product has to be used for research purposes only. 

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