Human Anti-15 Keto Cholestane (Oxidized Cholesterol)Technology #2017-204-170507
Questions about this technology? Ask a Technology Manager
Anti-15 Keto Cholestane HU #170507
PRODUCT NAME Anti – 15
Keto Cholestane Antibody
INFOMATION Oxysterols represent a family of lipids implicated in
diverse human diseases where Mab-based detection assays could have a profound
effect on their utility as clinical biomarkers. The antibody is targeting a
lipid implicated in multiple sclerosis and Autoimmune Encephalomyelitis (EAE)..
This antibody may be used for utilizing phage display to isolate highly
specific antibodies against poorly immunogenic, small molecule lipids.
DESCRIPTION This antibody is suitable for lipids and glycolipids
study. It is highly specific for 15- Keto Cholestane and shows little or no
binding activity for other closely related oxystrols.
FORMAT 100µg of
lyophilized, purified antibody. Reconstitute to 100µl of distilled H2O for
1µg/µl. It contains no additives.
SPECIFICITY This antibody is highly specific for 15- Keto
Cholestane and shows little or no binding activity for other closely related
STARTING DILUTIONS ELISA: 1:100. Optimal dilution has to be determined by the
antibody can be kept at 4°C for up to 3 months and should be kept at -20°C for
long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should
be aliquoted before freezing for short-term storage (-20°C) or for long-term
storage (-80°C). For maximum recovery of product, centrifuge the original vial
prior to removing the cap. Further dilutions can be made in assay buffer.
STABILITY Minimum 1
year from reception date.
REFERENCES 1. Islam MO1,
Lim YT, Chan CE, Cazenave-Gassiot A, Croxford JL, Wenk MR, Macary PA, Hanson
BJ.Generation and characterization of a novel recombinant antibody against
15-ketocholestane isolated by phage-display. Int J Mol Sci. 2012;13(4):4937-48.
LIMITATIONS This product has to be used for research purposes only.