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Human chimeric anti-HLA-A0201/EBNA1 for Epstein Barr Virus

Technology #2017-201-170504

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PRODUCT NAME HLA-A0201/EBNA1 Antibody 

BACKGROUND INFOMATION Epstein-Barr virus (EBV) is a gamma herpesvirus that causes a life-long latent infection in human hosts. EBNA1 is a latent gene product expressed by EBV-associated tumors and peptide epitope derived from this can be targeted by CD8 Cytotoxic T-Lymphocyte (CTL) lines. This antibody allows direct visualization and quantification of its epitope in tumour tissues and cell lines. 

PRODUCT DESCRIPTION A highly specific and sensitive antibody for EBNA1 epitope of the Epstein Barr Virus. 

FORMAT 100µg of lyophilized, purified antibody. Reconstitute to 100µl of distilled H2O for 1µg/µl. It contains no additives. 

HOST Human Chimeric 

CLONALITY Monoclonal 

ISOTYPE IgG1 

REACTIVITY/ SPECIFICITY This antibody was produced in mouse against EBNA1 peptide FMVFLQTHI. Specific for human and mouse. Other species not tested. 

APPLICATIONS Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC) 

RECOMMENDED STARTING DILUTIONS If reconstituted in 1ml: IHC 1:500. Optimal dilution has to be determined by the user. 

STORAGE Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. 

STABILITY Miinimum 1 year from reception date. 

REFERENCES 1. Sim, ACN., Too, CT., Oo, MZ., and Macary, PA. (2013) Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies. Nature Science Report 3. 3232, 1-5. 

LIMITATIONS This product has to be used for research purposes only. 

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