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CapTip, A Small and Portable Solid Extraction Device

Technology #2016-268

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CapTip, A Small and Portable Solid Extraction Device

FUNG Fun Man; FENG Huatao; LI Fong Yau

Faculty of Science

Department of Chemistry

Industry Problem

Solid-phase extraction (SPE) is a sample preparation process by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.

Problems with current SPE in the market

Presence of filters, glues, polymers in current SPE increase sample loss and contamination.

Solution

This technology relates to a portable and low cost device for solid phase extraction. It allows the use of commonly available micro-pipettes for on-site solid phase extraction of analytes, even if the analytes are present in very low concentrations.

CapTip enables high-fidelity chromatographic separation of even ultra-low concentration samples at 200 parts per billion (mass/volume).

The narrow aperture at the bottom of the CapTip permits liquid to pass through (with pressure via centrifuge or syringe), but retains chromatographic media in the tip. This eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Above all, the solid phase can be customized to suit the analyte of interest.


Figure 1: Schematic of how Solid Phase Extraction functions


Figure 2: Workflow - Analysis of LPS with CapTip SPE


Figure 3: Capillary Electropherograms of LPS: Commercial (Top); CapTip (Bottom), LPS extracted from Klebsiella pneumonia signal circled.

Value Proposition

•  Low cost and portable device

•  Enables high-integrity chromatographic separation of ultra-low concentration samples

•  Allows direct injection into the vacuum capsule should the need arises

Other Potential Application

•  This technology can be modified so as to extract and capture N-glycans from complicated samples.

•  Application of this technology to other analytes can be explored upon expression of interest.

For more information, contact:

NUS Industry Liaison Office

  :+65 6516 7175

  : iloquery@nus.edu.sg

  : enterprise.nus.edu.sg/commercialize

Ref : 2016-268

Principal Investigator: Prof. Li Fong Yau

Acknowledgement

This research is supported by the Singapore National Research Foundation under its Environment & Water Research Programme and administered by PUB, Singapore’s national water agency.