Synthetic Biology - Natural Large-scale Production of Short-Branched Chain Fatty Acids for the Chemical IndustryTechnology #2016-145
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Synthetic Biology - Natural Large-scale Production of Short-Branched Chain Fatty Acids for the Chemical Industry
Short branched-chain fatty acids (SBCFAs) are very useful platform chemicals that can be converted into many valuable industrial products like esters that are used for the production of plastics, plasticizers, surfactants and textile auxiliaries.
SBCFAs are mainly chemically synthesized. There is an increasing interest in producing these via environmentally-friendly and energy-efficient microbial biosynthesis routes to replace chemical synthesis. The budding yeast Saccharomyces cerevisiae has been found to natively produce three types of SBCFAs. However, the amount of SBCFAs generated in S. cerevisiae is low.
The invention is a method for overproducing SBCFAs in yeast (S. cerevisiae) cells by the following:
i) Construction of promoter replacement cassettes with a strong constitutive promoter
ii) Overexpression of single SBCFA pathway gene to increase SBCFA production
iii) Combinatorial overexpression of multiple genes with key roles in the biosynthesis, competition and secretion of SBCFAs.
The genetic modifications lead to a 31.2-fold increase in the
titer of SBCFAs as compared to wild-type yeast
Environmentally friendly method to produce
The utility of this method is in industries in the chemical space - flavors and perfumes.
Available for licensing. PCT application filed in October 2017.
A/Prof. Matthew Chang
Radhika Das Chakraborty
ILO Ref No: 2016-145