Apparatus and Methods relating to High Speed Stimulated Raman Scattering MicroscopyTechnology #16086n
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Stimulated Raman scattering (SRS) microscopy is an advanced label-free nonlinear optical imaging method based on intrinsic molecular vibrational contrast. Conventional point-scan SRS imaging suffers from relatively high excitation power density and low imaging speed. Hence, live cells may be easily damaged by the relatively high excitation power density. Furthermore, fast imaging is important because it allows observation of rapid molecular dynamics process in cells and tissues.
NUS researchers have developed a lock-in detection free line-scan stimulated Raman scattering (SRS) imaging technique based on a linear detector with large full well capacity controlled by a field-programmable gate array (FPGA). This lock-in free line-scan SRS imaging technique can achieve video-rate imaging with 20 times lower excitation power density than conventional point-scan SRS imaging. The FPGA can directly process the signal detected by the linear detector to obtain the SRS image. The rapid communication speed between the FPGA and linear detector allows using a linear detector array with a high line rate to increase imaging speed and to reduce 1/f laser noise, making it highly suitable and valuable in imaging the molecular dynamic process in live cells.
Fig. 1: Schematic diagram of the lock-in free line-scan SRS imaging technique based on an FPGA controlled linear detector. AOM, acousto-optic modulator; L, lens; M, mirror; DM, dichroic mirror; MO, microscope objective; CL, cylindrical lens; F, filter; PBS, polarizing beamsplitter; QWP, quarter-wave plate; LD, linear detector; OPO, optical parametric oscillator; FPGA, field programmable gate array; SRS, stimulated Raman scattering.
Fig. 2: Line-scan SRS image at 2940 cm-1 (CH3 stretching of proteins) of living gastric cancer cells during EMT processes induced by TGFβ1 using the excitation powers of Ipump of 0.3 mW/μm2 and IStokes of 30 mW/μm2 and imaging speed of 20 frames/sec. EMT, epithelial-mesenchymal transition; TGFβ1, transforming growth factor
• Low excitation power density
• Reduced 1/f noise and increased SRS imaging speed
• Effective dynamic imaging of unstained live cells and tissues in biological and biomedical systems
For more information, contact:
NUS Industry Liaison Office, Mr HJ Chen
:+65 6601 2814
Ref : ID16086N