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Hybrid Raman Spectroscopy and Optical Coherence Tomography Technique for Real-time In Vivo Tissue Measurements

Technology #16083n

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Zhiwei HUANG
Managed By
HJ Chen (
Manager (65)66012814
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Tech Offer 16083N Hybrid Raman [PDF]

Industry Problem

Raman spectroscopy (RS) is an optical vibrational technique based on inelastic light scattering process to reveal constituent-specific (e.g., DNA, proteins, lipids, and water) biochemical/biomolecular information about tissue and cells. In contrast, optical coherence tomography (OCT) detects the changes of tissue scattering, providing 2D/3D images of tissue morphology at submicron scales. RS and OCT provide different yet complementary information based on their different working mechanisms. OCT facilitates tissue morphology visualization whereas RS is capable of probing biochemical/biomolecular structures and conformations associated with disease transformation which cannot be obtained using OCT. Hence, combining RS with OCT technique would benefit better tissue diagnosis and characterization in biomedical systems.


NUS researchers developed a unique side-viewed handheld hybrid Raman spectroscopy (RS) and optical coherence tomography (OCT) technique for real-time in vivo tissue measurements. A side-viewed handheld RS-OCT optical probe is designed to co-align the optical paths of RS and OCT sampling arms, whereby a compact long-pass dichroic mirror (LPDM) is utilized to transmit the OCT signal through a gradient index rod lens and a reflection mirror. The LPDM deflects the tissue Raman signal by 90°, leading to co-aligned RS/OCT optical samplings on tissues. The hybrid RS and OCT technique developed is capable of simultaneously uncovering both the morphological and biochemical information about oral tissue in vivo, facilitating real-time in vivo tissue diagnosis and characterization at endoscopy.


 Value Proposition

•  Compact hand held RS and OCT endoscope probe

•  Provide both the high resolution (~9 μm) tissue morphology imaging, as well as FP/HW Raman spectrum with high spectral resolution (~ 9 cm-1) simultaneously and in real-time in vivo

•  Enable endoscopists to identify tissue lesions with higher sensitivity and specificity

 For more information, contact:

NUS Industry Liaison Office, Mr HJ Chen

:+65 6601 2814



Ref : ID16083N