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Controlled Mammalian Cell Culture with an Inorganic Thin-Film Coated Substrate

Technology #15151n

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Managed By
Ms Yong Yoke Ping (iloyyp@nus.edu.sg)
Manager (65)66011680
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15151N_Tech_Offer.pdf [PDF]

Market Opportunity

The ability to tailor enhancement and inhibition of cellular interactions on inorganic surfaces has not only important for scientific understanding, but for in-vitro (cell culture and flow cytometry/ microfluidic) and in-vivo (implants or device utilization) applications as well. Weak cell-surface adhesion to substrates is a common problem and presents technical limitations to the design of experiments; on the contrary, extensive adhesion of cells to surfaces that that should be free of cells (for example on retinal implants, heart valve implants, etc.) is also a problem.

Technology

This technology relates to an inorganic thin film coating which can be applied onto glass surfaces (such as glass slides,  coverslips, implant surfaces, etc), thereby enabling the  to direct/enhancement / inhibition of mammalian cell adhesion, proliferation and differentiation.



Figure 1: Primary human keratinocytes adhere onto the whole control wafer. But on the coated substrate (with varying composition), keratinocytes begin to adhere around the substrate coated at a certain composition.

 

Figure 2: Primary human fibroblasts adhere onto the whole control wafer. But on the coated substrate (with varying composition), fibroblasts begin to adhere on the substrate coated at a certain composition


Figure 3: Increase in mature lipid forming differentiated adipocytes is observed on the coated wafer as opposed to the control wafer without coating

Stage of Development

TRL 4. Component validation in laboratory environment

Applications

Enhance/inhibit specific mammalian cell adhesion, proliferation, differentiation; Coating of microfluidic devices; Coating to aid long term experiments; Study of different cellular properties by varying composition of coating

Advantages

1.  Culture of cells which are difficult to grow.

2.  Ability to control cellular processes such as adhesion, proliferation, differentiation

3.  Coating on materials to prevent or hinder growth of certain cell types.

Status

Patent pending. Available for licensing.