Mouse Anti- HepaCAM (Clone 5A1F1)Technology #14184n
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PRODUCT NAME Anti – HepaCAM
HepaCAM (GlialCAM) is frequently deleted in carcinomas, and reintroduction of hepaCAM intotransformed cell lines reduces cellular growth and induces senescence. Mutationsin HEPACAM give rise to the neurodegenerative disease megalencephalic leukoencephalopathywith subcortical cysts (MLC) since mutated hepaCAM prevents shuttling of MLC1 protein toastrocytic junctions in the plasma membrane. The study of the cytoplasmic domain ofHepaCAM may be used for evaluating the potential predictive and correlative functions inpreclinical and clinical studies of carcinomas and may assist in answering certain crucialquestions that may be useful for the study of Cancer.
This antibody is a monoclonal antibody that recognises the cytoplasmic domain ofHepaCAM.
100μg of lyophilized, purified antibody. Reconstitute to 100μl of distilled H2O for 1μg/μl. Itcontains no additives.
This antibody is highly specific for the cytoplasmic domain of the CD137 ligand.
Immunoflourescence (IF), Western Blot (WB), Immunoprecipitate (IP)
RECOMMENDED STARTING DILUTIONS
IF / IP: 1:1000. WB: 1:500. Optimal dilution has to be determined by the user.
Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°Cfor long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should bealiquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C).For maximum recovery of product, centrifuge the original vial prior to removing the cap.Further dilutions can be made in assay buffer.
Minimum 1 year from reception date.
Wu M, Moh MC, Schwarz H. HepaCAM associates with connexin 43 and enhances itslocalization in cellular junctions. Sci Rep, 2016 Nov 7;6;36218
This product has to be used for research purposes only.