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Mouse Anti-CD137 Ligand (Clone 1C8E9)

Technology #14175n

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Dr Esther Lee (
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CD137L dendritic cells induce potent response against cancer-associated viruses and polarize human CD8+ T cells to Tc1 phenotype
Cancer Immunol Immunother , 2018, Mar 5
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Anti_CD137L_MS_1C8E9.pdf [PDF]


Anti – CD137 Ligand 


CD137 and CD137 ligand interaction is important in transplant rejection, pathogen 
clearance and protection against malignant diseases. The study of CD137 ligand may 
be used for evaluating the potential predictive and correlative functions in oncology in 
preclinical and clinical studies and may assist in answering certain crucial questions that 
may be useful for the study of Cancer. 


This antibody is a monoclonal antibody that recognises the cytoplasmic domain of the 
CD137 ligand. CD137 ligand is expressed in B cell lymphoma and myeloma cell line. 


100μg of lyophilized, purified antibody. Reconstitute to 100μl of distilled H2O for 1μg/μl. It 
contains no additives. 






Human IgG1 


This antibody is highly specific for the cytoplasmic domain of the CD137 ligand. 


Immunoflourescence (IF), Western Blot (WB), Immunoprecipitate (IP) 


IF / IP: 1:1000. WB: 1:500. Optimal dilution has to be determined by the user. 


Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°C 
for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be 
aliquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C). 
For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Further dilutions can be made in assay buffer. 


Minimum 1 year from reception date. 


Dharmadhikari B, Nickles E, Harfuddin Z, Ishak NDB, Zeng Q, Bertoletti A, Schwarz H. 
CD137L dendritic cells induce potent response against cancer-associated viruses and 
polarize human CD8+ T cells to Tc1 phenotype. Cancer Immunol Immunother 2018, Mar 5. 

Rajendran S, Ho WT, Schwarz H. CD137 signaling in Hodgkin and Reed-Sternberg cell 
lines induces IL-13 secretion, immune deviation and enhanced growth. Oncoimmunology 
2016 Apr 29; 5(6). 

Harfuddin Z, Dharmadhikari B, Wong SC, Duan K, Poidinger M, Kwajah S, Schwarz H. 
Transcriptional and functional characterization of CD137L-dendritic cells identifies a 
novel dendritic cell phenotype. Sci Rep 2016 Jul 19;629712 

Dharmadhikari B, Wu M, Abdullah NS, Rajendran S, Ishak ND, Nickles E, Harfuddin Z, 
Schwarz H. CD137 and CD137L signals are man drivers of type 1, cell-mediated 
immune responses. Oncoimmunology 2015 Nov 11; 5(4) 
Shao Z, Harfuddin Z, Pang WL, Nickles E, Koh LK, Schwarz H. 
Trogocytic CD137 transfer causes an internalization of CD137 ligand on murine APCs 
leading to reduced T cell costimulation. J Leukoc. Biol. 2015 May; 97 


This product has to be used for research purposes only.