Mouse Anti-CD137 Ligand (Clone 1C8E9)Technology #14175n
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Anti – CD137 Ligand
CD137 and CD137 ligand interaction is important in transplant rejection, pathogen
clearance and protection against malignant diseases. The study of CD137 ligand may
be used for evaluating the potential predictive and correlative functions in oncology in
preclinical and clinical studies and may assist in answering certain crucial questions that
may be useful for the study of Cancer.
This antibody is a monoclonal antibody that recognises the cytoplasmic domain of the
CD137 ligand. CD137 ligand is expressed in B cell lymphoma and myeloma cell line.
100μg of lyophilized, purified antibody. Reconstitute to 100μl of distilled H2O for 1μg/μl. It
contains no additives.
This antibody is highly specific for the cytoplasmic domain of the CD137 ligand.
Immunoflourescence (IF), Western Blot (WB), Immunoprecipitate (IP)
RECOMMENDED STARTING DILUTIONS
IF / IP: 1:1000. WB: 1:500. Optimal dilution has to be determined by the user.
Lyophilized antibody can be kept at 4°C for up to 3 months and should be kept at -20°C
for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be
aliquoted before freezing for short-term storage (-20°C) or for long-term storage (-80°C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Minimum 1 year from reception date.
Dharmadhikari B, Nickles E, Harfuddin Z, Ishak NDB, Zeng Q, Bertoletti A, Schwarz H.
CD137L dendritic cells induce potent response against cancer-associated viruses and
polarize human CD8+ T cells to Tc1 phenotype. Cancer Immunol Immunother 2018, Mar 5.
Rajendran S, Ho WT, Schwarz H. CD137 signaling in Hodgkin and Reed-Sternberg cell
lines induces IL-13 secretion, immune deviation and enhanced growth. Oncoimmunology
2016 Apr 29; 5(6).
Harfuddin Z, Dharmadhikari B, Wong SC, Duan K, Poidinger M, Kwajah S, Schwarz H.
Transcriptional and functional characterization of CD137L-dendritic cells identifies a
novel dendritic cell phenotype. Sci Rep 2016 Jul 19;629712
Dharmadhikari B, Wu M, Abdullah NS, Rajendran S, Ishak ND, Nickles E, Harfuddin Z,
Schwarz H. CD137 and CD137L signals are man drivers of type 1, cell-mediated
immune responses. Oncoimmunology 2015 Nov 11; 5(4)
Shao Z, Harfuddin Z, Pang WL, Nickles E, Koh LK, Schwarz H.
Trogocytic CD137 transfer causes an internalization of CD137 ligand on murine APCs
leading to reduced T cell costimulation. J Leukoc. Biol. 2015 May; 97
This product has to be used for research purposes only.