Surface Modification of Silicone with Covalently Immobilized and Crosslinked Agarose to Inhibit Infection and Omental WrappingTechnology #13196n
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Surface Modification of Silicone with Covalently Immobilized and Crosslinked Agarose to Inhibit Infection and Omental Wrapping
Peritoneal dialysis; antifouling, antibacterial and
hemo-compatible coating for catheters
Exclusive/ non-exclusive licensing Partnership in commercial
ILO Ref: 13196N
Dr Cairan He (email@example.com) Industry Liaison Office, NUS Enterprise
Inventor: Prof. Koon Gee Neoh
Huge peritoneal dialysis market and beyond
FDA approved natural material (Agarose) and Heparin
Antibacterial effect increase by more than 100 times
In peritoneal dialysis (PD), the catheter, usually made of silicone, has been considered as the “lifeline” of the patient. However, PD catheter also serves as a nidus for bacterial infection. Furthermore, complications can result from fibrin deposition and omental wrapping of the catheter which obstructs the dialysate flow. In
this work, a crosslinked agarose (AG) polymer layer was
covalently grafted as a micron scale coating on silicone surface. This coating
reduced Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa
biofilm formation by more than 2 orders of magnitude. In addition, cell and
platelet adhesion and protein adsorption was also reduced by ≥ 90%. Without
compromising the antibacterial and antifouling property, further improvement in
hemocompatibility, as shown by the inhibition of platelet adhesion and
activation, prolonged plasma recalcification time and lower hemolysis degree,
was achieved by co-immobilization of
2.6 µg/cm2 of heparin (HEP) in the agarose coating. The AG-HEP coatings are not cytotoxic to mammalian cells, and are stable for extended periods in lysozyme aqueous solution and under autoclaving at 121 °C for 20 min.
The crosslinked AG coating of ~2 µm thickness is stable and maintains its antibacterial efficacy after 30 days aging in lysozyme solution and also after autoclaving. In addition, the AG coating can effectively resist non-specific protein adsorption and fibroblast and platelet adhesion. Co- immobilization of 2.6 µg/cm2 of HEP in the AG coating further improves hemocompatibility by inhibiting platelet activation, prolonging PRT and reducing the degree of hemolysis.
Concomitantly, the antibacterial and antifouling efficacy of AG is retained. The favorable antibacterial, antifouling and improved hemocompatible properties as well as non- cytotoxicity of the modified silicone offer promising opportunities for combating infection and omental wrapping of PD catheters.
The coating process has been demonstrated successfully and
is readily converted for scale up industrial use.
Data is available for demonstration to interested parties.
About the Inventors
Professor Koon Gee Neoh is a professor at Department of Chemical and Biomolecular Engineering National University of Singapore. She has received numerous prestigious awards and honors including Editorial Advisory Board for Langmuir and National Science Award (1996) etc.
Figure 1 Schematic illustration of the modification of silicone surface via oxygen plasma or ozone treatment (Step (1)), and UV or heat-induced immobilization and crosslinking of acrylated AG and methacrylated HEP (Step (2)).