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Synthetic Biology - Tool to Modulate Multiple Gene Expression Levels in Microbes

Technology #12264n

Synthetic Biology - Tool to Modulate Multiple Gene Expression Levels in Microbes


The engineering of microbes for production of useful chemicals, such as food additives, pharmaceuticals or biofuels is the main aim of synthetic biology. Modulating expression of multiple enzymes in a coordinated manner is the key technique used for optimizing yield for such applications. Current tools include generating randomized genetic knockouts and overexpression libraries, synthetic promoter libraries and tunable intergenic regions. Despite the availability of these tools, simultaneous optimization of the expression of a number of genes in a pathway is still highly empirical, unpredictable and time consuming.


The invention is a novel tool for the co-expression of multiple enzymes (genes) in pathways controlled by a single extrinsic factor. This results in a constant ratio of expressions when a single extrinsic factor is distributed over multiple promoters of different strengths.


  • Optimization of yield of a product of a multi-step enzymatic pathway in bacteria, yeast, insect or mammalian cell
  • Saves time by accurate modulation of four or more modules simultaneously
  • Performance of expression system predicted with a simple model
  • Demonstrated and optimized to work in the lycopene synthetic pathway and the amorphadiene synthetic pathway


The utility of this method is in industries such as energy and health (pharmaceuticals) for manipulation of genetic and metabolic pathways (synthetic biology and metabolic engineering applications).


Available for licensing. Patent pending in US and Singapore.


A/Prof. Too Heng Phon



Radhika Das Chakraborty

Phone: +65-66012768


ILO Ref No: 12264N